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For measuring temporal frequency thresholds, drift speed of the gratings (at 100%% contrast) was increased until tracking ceased.
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Nine different drift frequencies (0.5, 1, 2, 4, 6, 8, 12, 16 and 32 cycles/s) were used to measure the temporal frequency tuning.
Samples from Deadman Reach (Southeast Alaska), collected in 1989 and 2001, were used to measure temporal heterogeneity in allele frequencies.
We measured temporal prediction abilities, sensorimotor synchronization accuracy and neural entrainment to auditory rhythms as captured using an EEG frequency-tagging approach.
Flicker thresholds were measured at two temporal frequencies, 4 and 14Hz.
Using the virtual-reality optomotor setup (Prusky et al. 2004), we measured visual acuity, contrast sensitivity and temporal frequency thresholds of the optomotor reflex in both rl−/− and WT mice (Fig. 4).
Sections were rinsed extensively before being mounted in Aqua Poly-Mount (Polysciences, Inc .. Optomotry Using the virtual-reality optomotor system developed by Prusky et al. (2004) we measured visual acuity, contrast sensitivity and temporal frequency thresholds of the optomotor reflex of rl−/− and WT mice.
The stimulus designed to measure magnocellular visual function was a low spatial frequency Gaussian blob, flickered sinusoidally at a temporal frequency of 8.33 Hz.
We then used computer-controlled presentations of Gabors to measure the neuron's tuning for direction of motion (12 directions), spatial frequency (10 frequencies), and temporal frequency (10 frequencies).
A temporal frequency of 5 s−1 was chosen for the expanding stimulus because it elicits a maximum turning response as measured in a recent behavioral study [22].
In the Drosophila experiments described here, we therefore swept the temporal contrast of the 'probe' stimuli through a range of values (0 69%) and measured responses to these stimuli both in isolation, and in the presence of a 30% contrast mask at a different temporal frequency.
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