Sentence examples for measuring surface expression from inspiring English sources
First, we determined the activation of NK (pan-NK+ TCRβ−) and NK T (pan-NK+ and TCRβ+) cells by measuring surface expression of the activation marker CD69 by flow cytometry.
We next determined the activation status of APCs by measuring surface expression of the costimulatory molecules CD80 (Figure 3) and CD86 (data not shown) on macrophages (CD11b+CD11c−) and DCs (CD11c+) at day 4 PI.
Initial experiments measuring surface expression of DC activation markers revealed limited changes only.
The inhibitory effect of APOSEC on platelets was further characterized by measuring surface expression of different platelet activation markers.
To examine the downstream effect of TLR2 stimulation on CIITA, we measured surface expression of MHC class II, whose expression is regulated by, and depends on, CIITA.
To measure surface expression of Bdel1 and WT channels, we labeled each α1 subunit at the N-terminus with GFP, expressed them in HEK-293 cells with α2δ1 and β2a, then used confocal microscopy to quantitate GFP signal at the plasma membrane as described previously [27].
As a signature of release of cytolytic granules, we measured surface expression of CD107a.
We next measured surface expression of glucocorticoid-induced TNFR-related protein (GITR), a surface receptor upregulated on newly activated T cells.
Furthermore, we measured surface expression of the platelet granule membrane protein CD62P as well as the adhesion of platelets to leukocytes as markers of pro-inflammatory platelet function [ 20, 21].
(B ) Stg chimeric mice (1 × 10 CD44lo TGF-βRII+/+ CD4-cre+ (WT) or TGF-βRIIf/f CD4-cre+ (KO)) were infected with LCMV Armstrong and 3 days p.i., spleens were fixed immediately in 2% PFA and stained with antibodies to measure surface expression of CD25, Ly6C, and intracellular phosphorylation of STAT5 (pSTand) and pS6 directly ex vivo.
The effects of anthroposophical medications on DC maturation were determined by measuring surface receptor expression (anti-human CD14, CD83 and CD86 mABs; all from ebioscience, Frankfurt, Germany) using live cell gating in flow cytometric analysis.
