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To study the feasibility of measuring root surface area (RSA) by 3D scanning technique and cone beam computed tomography (CBCT) reconstruction in vitro.
Lin and coworkers reported that studies measuring root surface caries are quite uncommon in China [ 51].
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Five plants were sampled randomly in each subquadrate to measure the tensile strength of roots in the field, and another ten plants to measure root length, root surface area, and number of root furcations in the laboratory.
Together, these measurements give a quantifiable way to measure root morphology on the agar surface (Grabov et al. 2005).
The particle size measurement showed that particles are within the range of 20 50 nm and measured root mean square roughness of coated surface was around 10 nm.
Irrigation had no impact on any other measured root parameter.
However, the measured root total conductances did not differ significantly.
We did however not measure root extension.
We measured adventitious root number, root surface area, aerenchyma formation, ethylene production and the expression of aerenchyma-formation- and N-related genes by aeration treatment of each cultivar individually.
Root length and root surface area were measured using WinRHIZO (EPSON 1680, WinRHIZO Pro2003b, Regent Instruments Inc., Quebec, Canada).
After 24 h of incubation at 20°C, the plants were harvested, washed and the root surface areas were measured using WinRhizo®, as described above.
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