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Activation of T lymphocytes in human mononuclear cell cultures was monitored by measuring release of IFNγ in response to SEB pre-incubated with each antibody.
Heart damage was quantified by measuring release of cardiac troponin 1 into the serum of mock-infected or infected mice at days 3 and 7 PI using a commercially available ELISA kit (Life Diagnostics, West Chester, PA) according to manufacturer's instruction.
LDH assay determines membrane integrity by measuring release of LDH in the culture medium.
The α-glucosidase activity was determined by measuring release of p-nitrophenol from p-nitrophenyl α- D-glucopyranoside [ 39].
DNA fragmentation was assessed by measuring release of nucleosomal fragments into the cytosol (Cell Death Detection ELISA Plus, Roche).
To quantify their respective efficiencies, we used a coupled enzyme assay, measuring release of CoA following consumption of d-CoA (see Methods).
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A nitric oxide microsensor was immediately used to measure release of NO (both concentration and flux).
In addition, this could allow measured release of incorporated drugs or peptides using oscillating magnetic fields, creating highly diverse therapeutic potential for the synthesized system [27, 32]. Figure 4 Cellular uptake of magnetic nanospheres by PC12 cells.
The assay measures release of cytoplasmic lactate dehydrogenase in the culture medium as a result of cell lysis.
To measure release of Aβ species, each slice was transferred to one well of a 24-well plate immediately after preparation.
The assay measures release of hemoglobin spectrophotometrically.
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