Sentence examples for measuring protein expression from inspiring English sources

GeneChip analysis of the right ventricle was confirmed by qPCR for a subgroup of genes and was further substantiated by measuring protein expression showing a marked upregulation of tTG due to right ventricular hypertrophy.

Therefore, these plant extracts need to be further investigated by measuring protein expression of key transcription factors like peroxisome proliferator-activated receptor gamma (PPARγ) in both in vitro and in vivo models.

The primary objective of the trial was to determine the pharmacodynamic effects of a 4-week course of lapatinib administered preoperatively to patients with HER2-positive DCIS measuring protein expression of phospho extracellular-regulated kinase (pERK), phosphor-AKT (pAkt) and proliferative/apoptosis markers at baseline and post treatment.

Others have studied HIF-1α in breast carcinoma by measuring protein expression by immunohistochemistry. Zhong and colleagues found that only 29% of primary breast carcinomas (15 of 52) expressed HIF-1α protein, compared with 69% with lymph node metastasis [ 9], whereas Bos and colleagues reported that 80% of their invasive breast tumours (32 of 40) expressed HIF-1α protein [ 10].

While various methods have been developed for Escherichia coli and applied to fusions of promoters and Green Fluorescent Protein (GFP) [ 4, 8, 9], the few published examples of measuring protein expression using a fluorescence reader in other organisms such as yeast have been limited to highly expressed proteins (for instance [ 10]).

The benefit of using CAI is that it can be computed for any gene with a codon frequency distribution, whereas experimentally measured protein expression or ribosomal loading data are often limited to relatively highly expressed proteins or transcripts.

Additionally, immunostaining was used to measure protein expression.

Such techniques are valuable to measure protein expression, solubility, activity, and native posttranslational modification events in heterologous systems.

It is majorly used to measure protein expression levels and the intensities of specific bands by using an online software tool (Gelquant.NET).

However, the ability to measure protein expression, produce proteins (usually in recombinant form) and to block receptor ligand interactions remains essential for elucidating the function of the molecules of interest.

The term protein expression is however very rarely used in this sense, but tends rather to be used in the context of translation or protein synthesis, which is semantically not correct and further leads to misunderstandings when attempts are made to measure protein expression by two-dimensional gel electrophoresis or liquid chromatography.