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Crucial to this effort, the CPTAC network of laboratories demonstrated high reproducibility of this approach within and between laboratories as well as across instruments in measuring protein concentrations in plasma [38].
Reverse phase protein arrays (RPPAs) are a powerful high-throughput tool for measuring protein concentrations in a large number of samples.
The mitogenic effect of the compounds was also verified by measuring protein concentrations of the samples to reflect an increase in cell number.
This review focuses on fluorescence imaging methods that offer a quantitative description of DNA repair in single cells by measuring protein concentrations, diffusion characteristics, localizations, interactions, and enzymatic rates.
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After measuring protein concentration using the Bradford assay kit (Bio0Rad), lysates were subjected to ELISA.
A 5 µl portion of the extracted ER protein fraction was used for measuring protein concentration with the BCA™ Protein Assay Kit (Pierce , USA.
After measuring protein concentration (Bradford, BioRad, Hercules, CA), the cell lysate was diluted 1∶100 with 20 mM sodium phosphate, pH = 7.4 in a quartz cuvette under constant stirring at 37°C.
The protein elution was monitored by measuring protein concentration via Coomassie Plus Protein Assay Reagent (Pierce).
An important issue identified in the course of this work is that efficient, cost effective consensus methods for measuring protein concentration still do not exist [ 25].
BCA protein assay kit (Pierce, Rockford, IL) was used to measure protein concentrations as described in a previous report [36].
The Lowry assay was used to measure protein concentrations in a trichloroacetic acid (TCA) pellet (resuspended in 1 N NaOH) using bovine serum albumin as a standard.
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