Sentence examples for measuring protein concentration from inspiring English sources
After measuring protein concentration using the Bradford assay kit (Bio0Rad), lysates were subjected to ELISA.
A 5 µl portion of the extracted ER protein fraction was used for measuring protein concentration with the BCA™ Protein Assay Kit (Pierce , USA.
After measuring protein concentration (Bradford, BioRad, Hercules, CA), the cell lysate was diluted 1∶100 with 20 mM sodium phosphate, pH = 7.4 in a quartz cuvette under constant stirring at 37°C.
The protein elution was monitored by measuring protein concentration via Coomassie Plus Protein Assay Reagent (Pierce).
An important issue identified in the course of this work is that efficient, cost effective consensus methods for measuring protein concentration still do not exist [ 25].
The rest of the procedures for measuring protein concentration and PAGE (using 12.5% acrylamide gels or 5 20% acrylamide gradient gels) were performed similarly as described in " Cell culture, transfection, and immunoblot analysis of rCa v2.1 proteins".
Crucial to this effort, the CPTAC network of laboratories demonstrated high reproducibility of this approach within and between laboratories as well as across instruments in measuring protein concentrations in plasma [38].
Reverse phase protein arrays (RPPAs) are a powerful high-throughput tool for measuring protein concentrations in a large number of samples.
The mitogenic effect of the compounds was also verified by measuring protein concentrations of the samples to reflect an increase in cell number.
Following centrifugation at 12.5 K × g, BCA assay was used to measure protein concentration in collected supernatants.
Aliquots of the homogenates were used to measure protein concentration.
