Sentence examples for measuring phosphorylation of from inspiring English sources

To ensure that these differences were not limited to measurement at only a β-arrestin recruitment endpoint, equivalent studies were performed by measuring phosphorylation of ERK 1/2, and similar results were obtained (Fig. 1 E H).

Src kinase activity was determined by measuring phosphorylation of a synthetic derivative of p34cdc2, a specific substrate for src.

Cell extracts from control and SIRT1 knockdown were used for measuring phosphorylation of c-Jun kinase.

We then tested the in vitro kinase activity of p38 by using immune complexes containing p38 from aliquots from these same cells, measuring phosphorylation of the p38 substrate ATF2 (Figure 1A, bottom).

For that purpose, we developed a modified version of the previously described KIRA-ELISA [28], a rapid, sensitive and high-capacity assay that quantifies TrkB activation in ELISA microtiter plates by measuring phosphorylation of its tyrosine residues [see Data S1 and Fig. S1,S2].

We addressed CWI pathway induction by measuring phosphorylation of the MAP kinase Slt2p.

Additionally, we measured phosphorylation of Akt at Ser473.

We investigated the feasibility of RI detection techniques used to measure phosphorylation of a GBP-fused substrate, in turn facilitating highly sensitive detection of CaMKII, employing an LOC.

In addition, we measured phosphorylation of IRF3 which lies downstream from MPYS in the IFNβ stimulation pathway [16].

To complement our flow cytometric studies of mTOR activity, we measured phosphorylation of another protein, S6-kinase, by Western blot.

We also measured phosphorylation of AKT at S473, which is in part a marker of mTORC2 activity.