Sentence examples for measuring oxidation of from inspiring English sources

We tested the steady-state levels of ROS in these mutants by measuring oxidation of the dye 2',7'-dichlorodihydrofluorescein diacetate (DCFH-DA) (Figure 3B).

Complex IV (cytochrome c oxidase) activity was determined in 10 mM potassium phosphate (pH 7.4) with 0.1% reduced cytochrome c (reduced by sodium hydrosulfate) by measuring oxidation of reduced cytochrome c at 550 nm (extinction coefficient 19.0 mM/cm) [29].

The reaction was started by addition of 3-4 mM acetolactate and monitored by measuring oxidation of NADPH spectrophotometrically at 340 nm.

The reaction was started by addition of 2 mM KIV and monitored by measuring oxidation of NADH spectrophotometrically at 340 nm.

Briefly, complex I activity (NADH ubiquinone oxidoreductase) was determined by measuring oxidation of NADH at 340 nm (using ferricyanide as the electron acceptor).

Laccase activity was calculated by measuring oxidation of 5 mM ABTS in 25 mM succinate buffer (pH 4.5) at 436 nm, using the absorption coefficient of 29 300 M-1 cm-1 [ 46].

This effect along with the hydrophobicity of the fluorinated electrode resulted in significantly lower ClO4− formation (96% decrease) while slightly enhancing measured oxidation rates of hydrophobic organic compounds.

For kinetic calculations an assay measuring the oxidation of 6-MPH4 using a coupled reaction measuring the decrease in absorbance at 340 nm due to NADH oxidation (61) was used as this assay has been validated for use in several previous studies (20,25,66).

PPO activity was measured according to the method of Duan et al. [3] by measuring the oxidation of 4-methylcatechol.

The level of oxidative stress in L02 cells was determined by measuring the oxidation of DCFH-DA (Beyotime Company, Shanghai, China) as previously described.

PEPCK enzyme activity was measured through the oxaloacetate formation from PEP, with NaHCO3 serving as the source of CO2 and was determined by measuring the oxidation of NADH in the presence of malate dehydrogenase (MDH) at 340 nm.