We have added representative pictures and have highlighted that cell alignment was quantified by measuring nuclei orientation.
We selected one to three sections from two to four tumours of each group, and systematically measured nuclei within the cross-points of the 200 × 154 μm grid, starting at the top left corner and continuing to the right bottom corner of the grid.
We next assembled plots that, for each cell line, display the boundary curvatures of all of the measured nuclei, as shown in Figure 1c.
To elucidate if the nucleus size played a role in determining the cell's invasive behavior, we measured the nucleus diameters of cells, with respect to SPC treatment, on a flat surface.
To measure the nucleus orientation, confocal pictures of serial sections of E12.5 midbrains were taken with an optical slice of 1 µm.
Furthermore, some important traits such as disease resistance cannot be measured in nucleus lines.
(b) DAPI was used to analyse the number of cells per image and to measure the nucleus size of the cells.
In synapsis-defective mutants, distances were measured in nuclei that occupied similar positions in the gonad as mid and late pachytene nuclei in wild-type animals.
Because neuronal cells contain only a single nucleus, measuring the DNA content in each dish provides a measure of cell number.
On this foundation we have developed a demonstrative analysis tool capable of measuring kinetoplast and nucleus DNA content, size and position and cell body shape, length and width automatically.
