Sentence examples for measuring mRNA expression from inspiring English sources

Many of the applications of real-time Q-PCR include measuring mRNA expression levels, DNA copy number, transgene copy number and expression analysis, allelic discrimination, and measuring viral titers.

A multi-marker panel measuring mRNA expression of CCN1, EGFR, FGF19, FRS2, and GREB1 in saliva, differentiated patients with lung cancer from controls with a sensitivity of 93.75 % and specificity of 82.8%[46]6].

BSA was first combined with microarray genotyping in yeast using arrays designed for measuring mRNA expression [2].

Based on the high resolution type, we optimized allele-specific real time-PCR for measuring mRNA expression of six HLA alleles: A*02 01 01, A*03 01 01, B*07:02 01 and B*27:02, C*02 02 02 and C*07:02 (Materials and methods).

Compared to cytosolic RNA, the nuclear fraction is less well suited for measuring mRNA expression levels.

Finally, we used HTSeq [ 52] along with watermelon gene annotation file from the Curcurbit Genomics Database for measuring mRNA expression.

When preceded by reverse-transcription PCR, qPCR is a powerful tool to measure mRNA expression and is the gold standard for microarray gene expression data confirmation.

Semiquantitative reverse transcriptase-polymerase chain reaction was performed to measure mRNA expression of the amphotropic retroviral receptor.

We measured mRNA expression and protein levels in candidate immune (TNF-α, IL-1β, IL-10, IFN-δ) and neurotrophic (brain-derived neurotrophic factor (BDNF)) markers from plasma.

Next, we measured mRNA expression patterns using DNA microarray over time in the cell-free fraction of blood samples in mice after one hour ischemia followed by reperfusion of the left anterior descending (LAD) artery.

We sought to derive and externally validate a multigene expression assay prognostic of overall survival (OS) that is superior to clinicopathologic variables in patients with surgically resected CRLM.We measured mRNA expression in prospectively collected frozen tumor from 96 patients with surgically resected CRLM at Memorial Sloan Kettering Cancer Center (MSKCC, New York, NY).