Sentence examples for measuring luc activity from inspiring English sources

Protein production was estimated by measuring luc activity.

The translation of C+Luc mRNA directly transfected into uninfected cells was quantified by measuring luc activity at 90 minutes post-electroporation (mpe).

At 1, 3 or 5 hours after the second electroporation cultures were collected and luc production was determined by measuring luc activity (Fig 1C) and also by western blot analysis employing anti-luc antibodies (Fig 1C).

To ask if host transcription was required to resist RNA virus infection, we treated VSV-LUC and SINV-LUC-infected LD652 cells with increasing doses of actinomycin D (ActD) (Black and Brown, 1968) and then measured LUC activity 48 hpi.

The samples used to measure luciferase (Luc) activity were recovered in luciferase lysis buffer (see below), whereas sample buffer was added to the replicates to be analyzed by SDS-PAGE followed by Western blotting.

Entry as measured by Luc activity was higher for IHD-J than WR at neutral pH; however, entry of IHD-J was neither accelerated by brief low pH treatment nor inhibited by bafilomycin A1 [12].

In the present study, we found that VACV entry into S2 cells, measured by Luc activity, was enhanced less than 2-fold by brief exposure of attached virions to pH 5 buffer compared to 8-fold enhancement of entry into HeLa S3 cells (Fig. 3A).

We expressed hTrpC3, which is insensitive to rat TrpC3-shRNA, in the presence of rat TrpC3-shRNA and subsequently measured BNP-Luc activity.

Luc activity measured in cells electroporated with IRES-Luc increased consistently throughout the experimental period, irrespective of 2Apro-expression and eIF4G cleavage (Fig 1A).

COS-1 cells were infected by serial dilutions of the reaction mixture, LUC activity was measured 2 days later, the data plotted and TU titer was determined by extrapolation to null LUC activity.

Luc activity was measured with Luc assay reagent (Promega) using a Turner TD-20e Luminometer (Sunnyvale, CA) and β-gal activity was measured using β-gal assay reagent (Promega) and reading the absorbance at 420 nm in a spectrophotometer.