A radiologist interpreted MBI studies in a randomized, blinded fashion to assign an assessment score (1 5) and measured lesion size.
Our method to measure lesion size has been previously reported [19].
The whole plant inoculation assays were adapted to quantitatively analyze fungal growth in planta, whereas the detached leaf inoculation assays were used to quantitatively measure lesion sizes.
In a model of experimental liver metastases in rats, we measured lesion sizes after interstitial illumination of tumours at different times after intravenous administration of BCA (10 mg kg -1) bodyweight), as well as BCA concentrations in liver and tumour tissue.
However, the recommendation of the authors of the RECIST group criteria was followed in measuring lesions no smaller than twice the size of the slice thickness [ 3].
In the present study, further analysis of transplanted animals demonstrated no changes in the host microenvironment as measured by lesion size and tissue sparing, serotonergic regeneration/sprouting, NG2 deposition, glial scarring, and angiogenesis as measured by stereology.
The final output is a numerical value, which corresponds to the combined area of detected pixels (expressed as millions of pixels, Mpx), which in turn can be used to define a cut-off value for a positive diagnosis and to measure the lesion size.
We measured the lesion size at the level of photoreceptor inner segments (IS) or, in class 2 lesions, at the outer nuclear layer (ONL).
Progressive disease (PD) was defined as the unequivocal appearance of any new lesion or an increase of ⩾25% in the sum of the perpendicular diameters of any measured lesion or in the estimated size of a not measurable lesion.
Disease in the detached leaf inoculation assays was estimated by measuring the lesion sizes.
