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The ELISA procedure of measuring lactoferrin was as described previously [ 43].
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The total iron content in lactoferrin was measured by the application of ICP-MS.
A search of the medical literature revealed that highly elevated lactoferrin was closely correlated with Crohn's and ulcerative colitis.
Lactoferrin was purchased from Seebio Company (Shanghai, China).
The plasma clearance half-life of lactoferrin was increased 5- to 20-fold by this modification.
This effect was abolished when iron-saturated lactoferrin was replaced with unsaturated lactoferrin.
Lactoferrin was detected in both non stimulated and LPS stimulated samples.
Furthermore, lactoferrin was associated with renal and hematologic organ failure.
To obtain homogeneous lactoferrin preparation, the MonoS elution fraction containing the non-glycosylated variant of lactoferrin was pooled, iron-saturated/desaturated, and subjected to re-chromatography (Fig. 2c).
Human lactoferrin was found to be saturated in 20 % [ 26], while bovine lactoferrin is saturated with iron in 15 19 % [ 27, 28].
Recombinant human lactoferrin (lactoferrin) was produced at Fujifilm Diosynth using Aspergillus niger as the expression system and purified by cation exchange chromatography.
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