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The Hex, a lightweight device that straps onto a user's thigh, determines muscle oxygen levels by emitting light into the muscle tissue and measuring its absorption, in a process called near-infrared spectroscopy.
The quantitative determination of MO was performed by measuring its absorption with a UV vis spectrophotometer (PerkinElmer Lambda-35).
The reduced nicotinamide-adenine dinucleotide formed was determined by measuring its absorption at 340 nm against a blank preincubated and incubated as above, but to which water was added instead of acetaldehyde.
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This was explored in another laboratory exercise in which students used bromothymol blue as a blood analogue and measured its absorption spectrum using a low-cost spectrograph (Vernier, www.vernier.com/products/sensors/spectrometers/svis-pl).
Esophageal acid exposure was determined using standard pH monitoring, and duodenogastroesophageal reflux was measured by a spectrophotometric method (Bilitec) that identifies bilirubin in the refluxate by measuring its characteristic light absorption spectrum.
The dough was further evaluated by measuring its strength and water absorption.
Each isoform concentration was calculated after measuring its cadmium content by atomic absorption spectrometry with inductively coupled-plasma.
The concentration of IR700 was measured by its absorption to confirm the number of fluorophore molecules conjugated to each Panitumumab molecule.
The tissue were initially deproteinized with Somogyi reagent [ 16] (using 2.0 ml 55 mmol/L NaOH followed by 2.0 ml 75 mmol/L ZnSO4), after centrifugation, aliquots of supernatant ONOO− concentration was measured from its absorption at 302 nm by using ϵ302 = 1670 molL-1cm-1[ molL-1cm-1[ molL-1cm-1[ 17
This involves measuring water absorption, its inhibition by mercuric chloride and subsequent restoration by certain reductants such as DTT.
It should be noted that when the same supernatant was measured for several times, its absorption did not change, indicating that no enzyme detached from the particles during the 2 min reaction time.
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