Sentence examples for measuring gene activation from inspiring English sources
For measuring gene activation, we employed LC-1 reporter mice [21], which harbor a bidirectional Tet-promoter cassette (Ptetbi) with genes for luciferase (Luc) and Cre recombinase (Cre) (Fig. 1A).
RNA collected from cells was used to measure gene activation of IL1RN, sgRNA expression levels and spacer sequence identities.
Biological activity of the stressed preparations was assessed in three different in vitro cell-based bioassay systems: a late-stage anti-proliferative assay and early-stage assays measuring reporter gene activation or endogenous gene expression by quantitative real time Reverse Transcription-Polymerase Chain Reaction (qRT-PCR).
Further, we have applied a lung-protective ventilatory strategy in the donor animals and during the reperfusion phase of transplantation and measured gene expression of important cytokines, chemokines and markers of endothelial activation throughout the protocol.
To verify differential transcriptional activation of genes in A. niger grown on SEB for 6, 12 and 24 hours, we measured gene expression using microarray procedures.
In all experiments measuring drug-induced gene activation, a negative control group (naïve) was included.
The focus was on tests based on receptor binding and receptor activation in cell cultures measured via reporter gene activation or cell proliferation: Competitive ligand-binding assays measure the binding affinity of a substance to an (isolated) receptor.
Cells were incubated for 5 days and an RPA was done to measure EBV lytic gene activation.
In general, the fish cells were more sensitive than rat cells to prooxidants, and the assays for lipid peroxidation and ARE reporter gene activation were more sensitive for measuring oxidative stress than GSH or %GSSG.
Here we report the establishment of an in vivo system that uses hsp70 gene activation as a measure of cadmium toxicity in living early larvae of transgenic zebrafish carrying a stably integrated hsp70-enhanced green fluorescent protein (eGFP) reporter gene.
Direct activation of estrogenic receptors (ERs) is detected photometrically by measuring luciferase activity, which allows quantitative and time-course analysis of target gene activation in vivo.
