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Morphometrical analysis was performed using the Image-Pro Plus 6.0 software (Media Cybernetics, Inc. Silver Spring, MD) and measuring five fields from each ear section and contrasting controls against challenged mice.
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We determined the mean thickness by measuring two fields from five sections per sample, and the entire depth of field was sampled, ignoring the upper and lower 0.5 μm.
We measured four fields and 1000 1200 cells per microscope slide.
The final value was determined based on measuring five random fields per section and three sections per kidney.
The magnetotelluric (MT) method uses the natural electromagnetic field variation to image subsurface resistivity structure, and usually involves measuring two horizontal electric field components (E x and E y ) and three magnetic field components (B x, B y, and B z ) at the Earth's surface, where the subscripts x and y indicate the N-S and E-W directions, respectively.
Quantification of apoptotic cells was by measuring three randomly selected microscopic fields (100× magnification) for each slide.
This analyzer measured ten random fields per slide and calculated the ratio of connective tissue to the whole area of the liver.
Four Pb-PbCl2 electrodes were used for measuring two orthogonal components of electric fields (N-S and E-W directions), and one additional electrode was used for grounding.
The precision of low-field MRI for measuring zero motion depends partly on the imaging technique and partly on the analysis process, which consists of segmentation and registration.
The average nuclear fluorescence intensity was measured in five fields of 50 cells by Metamorph.
Immunofluorescence intensity or immunoreactive area was measured in five fields (300 μm) for every section in at least 10 sections for every experimental group (http://imagej.nih.gov/ij/index.html free software).
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