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We conclude that microarrays remain useful and accurate tools for measuring expression levels, and RNA-Seq complements and extends microarray measurements.
However, current technology does not provide cheap methods for measuring expression levels of binding factors directly.
We confirmed that these cells were under ER stress by measuring expression levels of typical ER stress markers, spliced XBP-1 and BiP.
hESC differentiation was determined by measuring expression levels of NESTIN and PAX6 for ectoderm, T and DESMINE for mesoderm and GATA4 and SOX17 for endoderm.
To determine whether Wnt signaling plays a role in prostatic tumorigenesis and tumor progression, we assessed the Wnt signaling status in human prostate cancer cell lines and 2 human prostate tumor xenografts by measuring expression levels of Axin2.
The generation of cells of the pancreatic lineage was assessed by measuring expression levels of the following markers: IAPP, IGRP, insulin I and insulin II (beta cell markers), the transcriptional regulators Nkx 6.1 and PDX-1 (pancreas development markers) and an endocrine marker, PAX 6 using Q-PCR.
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To evaluate whether the expressed variants are important for community function, it will be necessary to measure expression levels over time in conjunction with process measurements.
To assess radiation-induced response of protein markers in human lymphocytes irradiated ex vivo, we measured expression levels of our three best candidates, FDXR, DDB2 and ACTN1 protein, 3 days after initial exposure (Fig. 4B).
We also measured expression levels of classical HLA genes in eight HLA homozygous reference cell lines at baseline and after three treatments (heat shock, LPS, and IFN-gamma).
Measured expression levels are all relative to these references.
We next used qRT-PCR to measure expression levels (Fig. 5).
More suggestions(15)
measuring protein levels
measuring cortisol levels
measuring blood levels
measuring glucose levels
measuring sound levels
measuring chromium levels
measuring expression changes
measuring inventory levels
measuring radiation levels
measuring sea levels
measuring melanin levels
measuring ubiquitin levels
measuring isoprostane levels
measuring job levels
measuring dopamine levels
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