Sentence examples for measuring enzyme velocity from inspiring English sources
To ensure that we are measuring enzyme velocity over physiologically relevant substrate concentrations, we applied quantitative immunoblotting (with a standard curve of recombinant protein) to measure the concentration of eIF2a in the cytosol of mammalian cells and calculated the same for yeast cells (based on information available in a public data base; Ghaemmaghami et al., 2003).
To confirm the exceptionally high potency of BAY-678 (20) and BAY 85-8501 (29), we measured enzyme reaction velocities with different substrate concentrations at various inhibitor concentrations and extrapolated the inhibition constants (Ki) from Dixon plots (Supporting Information).
In such experiments it is simplest to measure the rate of conversion of substrate to product over the early time points, before substantial substrate depletion has taken place, as later measurements may underestimate enzyme velocity.
Enzyme velocity, V was measured at substrate concentrations well below the enzyme's K m and in samples with less than 25% substrate depletion.
Enzyme stability was determined by measuring the enzyme activity (initial velocity) as a function of the incubation time (for up to 4 days) as follows: vials containing the enzyme (lyophilized powder: 10 mg, and co-lyophilized: 1.0 mg) suspended in 1.0 mL 1,4-dioxane under N2, were incubated at 45°C under constant shaking (300 rpm).
This tethered arrangement provides a very high effective substrate concentration that increases enzyme velocity (equation 2).
Segments of constant enzyme velocity were fitted with straight lines to extract translocation rates and processivities.
Momentum is mass times velocity, but all electrons weigh the same, so measuring their velocity was enough.Enough.
The spider's velocity was determined by measuring the average velocity of the spider's body on a run.
This strategy requires knowledge of maximal enzyme velocities (vmax).
Hence, when measuring muscle tissue velocities orthogonally projected, velocities are always relative.
