Sentence examples for measuring enzyme activities from inspiring English sources

The pH optimum was assayed by measuring enzyme activities at different pH levels (Figure 5A).

Kinetic parameters were analyzed by measuring enzyme activities against guaiacol, DMP and ABTS (as described above) over the concentration range 5 95 µM under the optimal assay conditions.

The protein content of each fraction used as an enzyme source was determined by the Bradford method before measuring enzyme activities.

They could prove their hypothesis by measuring enzyme activities.

The number of likely isozymes complicates the interpretation of results from traditional biochemical approaches, such as measuring enzyme activities in tissue extracts.

For the enzyme assays, the concentration of each compound that inhibited 50% of the enzyme activity (IC50) was determined by measuring enzyme activities in the absence and presence of increasing concentrations of inhibitor (ranging from 0.5 to 10,000 nM).

The optimum temperature was determined by measuring enzyme activity in a temperature range from 20 °C to 65 °C for 20 minutes, and the higher value observed was at 40 °C (Fig. 1D).

The optimum temperature of purified protease was determined by measuring enzyme activity at varied temperatures (30°C to 70°C).

The coenzyme preference of the recombinant AcCR was examined by measuring enzyme activity using 4′-chloroacetophenone or isopropanol as substrate.

All steps were carefully performed at 4°C, while a Jasco V-530 UV-vis spectrophotometer (Jasco Co., Midrand, Gauteng, South Africa) was used for measuring enzyme activity.

11βHSD1 protein in adipose tissue was quantified by measuring enzyme activity in the dehydrogenase direction, which is the preferred reaction in tissue homogenates with excess cofactor [20], [21].