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In a phenotypic and activity-based protein profiling (ABPP) screening for small molecules that show activity in a cell-based assay measuring differentiation and lipid accumulation in adipocytes, a subset of bioactive inhibitory compounds that target Ces1d was identified (Dominguez et al., 2014).
Cell differentiation was assessed by measuring differentiation antigen expression after 24 and 96 hours treatment with combined MPA and BEZ using flow cytometry (Becton Dickinson FACS Calibur and Becton Dickinson Cell Quest software) and PE-CD11b conjugated antibodies for HL-60 and KG1a cells.
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We generated embryoid bodies using the hanging drop method, and measured differentiation by analyzing the extent of the differentiated outgrowth upon plating the EBs.
It is employed to measure differentiation of features to topics in filtration.
A similar finding was observed when EB-derived CD34+ cells were co-cultured with OP9 cells, and the lymphoid marker, CD7, was used to measure differentiation into the lymphoid lineages.
We also used haplotype frequencies to measure differentiation, which has been shown to be more reliable than sequence diversity for smaller sample sizes [25] but results for both statistics were similar for antigens with smaller sample sizes (lsa1, glurp, pfs48/45).
The fixation index (F ST) can be calculated to measure differentiation between populations.
The fixation index (FST) can be calculated to measure differentiation between populations.
Antiangiogenic effect was further studied using HUVECs tube formation on matrigel matrix which measures differentiation of endothelial cells.
We then measured differentiation and divergence between phylogeographic groups using F ST [ 38] and D xy, the average number of nucleotide substitutions per site between groups [ 39].
Even though C. rubella harbors so much of its own unique polymorphism, this is not captured by the Fst statistic, which measures differentiation alone.
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