Sentence examples for measuring conformational changes from inspiring English sources

This makes them most suitable for measuring conformational changes in biomolecules such as nucleic acids since protein unfolding often requires forces >100 pN.

The signal-to-noise ratio for the Y274W-S96CCPM mutant was significantly lower than for the Y274W-S112CCPM mutand, and consequently, a second P2 phase could not be clearly distinguished, indicating that this FRET pair was not as well suited for measuring conformational changes.

The ECRET between luminol and QD was used to evaluate interactions between DNAs and to measure conformational changes of DNAs.

The far-UV CD is an excellent technique to measure conformational changes upon introduction of mutations in proteins; 222 is used as a probe to measure changes in α and β structure [17].

This approach allows us to measure conformational changes occurring in the primary phototransition of ChRs (ChR → P1) and compare the changes to those of other microbial rhodopsins, including ChR2 from Chlamydomonas reinhardtii (CrChR2).

Recent studies have shown, however, that the function of mutant p53 can be restored by exposing cells to small molecule compounds identified in a novel screen that used antibodies to measure conformational changes to the p53 protein [ 32].

FRET can also be used to measure conformational changes of large molecules and is increasingly used to read out genetically expressed biosensors for signaling molecules such as calcium [ 4], potassium [ 5], chloride [ 6], GTP [ 7], IP3 [ 8], PIP2 [ 9] and others [ 10].

An analogous approach has successfully been used to measure conformational change in the presence of heparin for antithrombin [12].

Techniques other than optical responses have been used to measure conformational change.

3) If the measured conformational change is indeed rate limiting then its temperature dependence should be the same as that of the ATPase activity measured before.

In this study, we aimed to determine the nature and location of PTM sites using mass spectrometry in parallel with assessment of the catalytic and force-generating capacity of specific myosin isoforms using a modified single fiber in vitro motility assay and measure conformational changes during single muscle fiber contraction using small-angle X-ray scattering.