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After measuring cells number, the titanium disk was divided into nine areas.
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In addition, for MCF-7 cells we verified the utility of this assay for measuring cell number by comparison with DNA content measurements and with cell counts by hemocytometer (data not shown).
Cells were treated with nine concentrations of drug for 72 h before measuring cell number relative to controls [12].
We first checked for defects in cell proliferation by measuring cell number in Drosophila S2 cells upon RNAi-mediated depletion of genes of interest.
The utility of the CV assay in measuring cell number was verified both in MCF-7 cells and in combination with immunodetection plate assays for GH3/B6 cells [ 17].
Methodologically, the activity of STI571 in combination in these studies was assessed by the MTT assay, which is strictly a technique for measuring cell number and not cell kill (Carmichael et al, 1987).
First, we studied the effect of BRAT1 silencing on cell growth by measuring cell number and the MTT (3- 4,5-dimethylthiazol-2-yl -2,5-diphenyltetrazolium bromide, a yellow tetrazole) assay.
In the RIF-1 tumours, these proliferation markers correlated significantly with each other as well as with the ΔT1, supporting the notion that ΔT1 is a surrogate of the remaining number of proliferating cells in a tumour after therapy even though our histological analysis suggests that it cannot be measuring cell number or density directly.
This study was found to be valuable in detecting polymicrobial infections; by measuring cell number, light scattering, and DNA content-associated fluorescence, the findings show that it was possible to detect susceptible and resistant cells within an hour of incubation with ampicillin in the same sample.
Bacterial colonization was monitored from 0 to 14 days in three concentric portions of the sterilized clods (outer, intermediate and inner) and in the surrounding non-sterile soil, by measuring cell numbers and substrate-induced respiration (SIR).
Therefore, we performed cell proliferation studies in CA9-22 by measuring cell numbers (Trypan Blue exclusion), DNA synthesis (H-thymidine incorporation), and PCNA expression (immunohistochemistry).
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