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Further investigation of the mechanism of GST-C translocation was performed by measuring cell uptake after treatment of cells with the known endocytotic inhibitors chlorpromazine, amiloride and filipin.
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For comparative purposes we also measured CFSE-cell uptake by the cervical lymph nodes.
An EdU uptake assay, which measures cells in S phase, revealed that control (ry ) ISCs show a peak in S phase at ZT6.
For measuring uptake, cells were incubated with [125I]IPP (0.1 microM) and cell-associated radioactivity was measured at various times.
Cell uptake was measured under normoxic and hypoxic conditions and in the presence and absence of 3 µM sunitinib.
Cell uptake, as measured by computed tomography and ICP-OES, as well as TEM images of cells, confirmed strong AuNP uptake for certain formulations, but there was no correlation with toxicity.
Moreover, a murine macrophage-like cell line, RAW 264.7 cells, was used to measure and image the cell uptake of fluorescent PLA/PNIPAAm PLA NPs at 30 °C and 37 °C on the boundary of LCST (34 °C).
Measures of relaxivity can track cell uptake and has been used to track the migration of cells to liver, kidney and spleen after injection [11].
Cell uptake data are expressed as percent of measured radioactivity per 1 mg protein (%radioactivity/mg protein).
Each cell uptake experiment was done in triplicate, and each replicate was measured five times by ICP-MS.
Fig. 8 Cell uptake study.
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