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Polymorphonuclear neutrophil activation was analyzed by measuring cell surface and soluble cell adhesion molecule expressions.
Immune responses were quantified by measuring cell surface marker expression with flow-cytometric analysis and mRNA levels with quantitative reverse transcriptase-polymerase chain reactions in whole blood before and after exposure.
We assessed class-switching of atypical MBCs by measuring cell surface expression of IgG by flow cytometry (Fig. 4).
In this context, measuring cell surface levels of MC1R is not an adequate estimation of the availability of this protein to the T cell system.
To evaluate neutrophil activation in the continuum of sepsis measuring cell surface receptors and oxidative metabolism; to evaluate monocyte activation measuring oxidative metabolism; and to evaluate the correlation between cell activation and organ dysfunction.
We provide evidence that cells sense a critical cell size by measuring cell surface area rather than, for example, cell volume or absolute length, a mechanism that could function regardless of the cell shape.
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Using fluorescently labelled anti-ErbB2 or anti-EGFR extracellular antibodies allowed us to measure cell surface ErbB2 and EGFR levels following POH1 knockdown by FACS analysis (Figure 7A,B).
We tested the effects of over expression of RACK1 in transiently expressing HEK cells by co-transfecting Flag-M2 with either PCDNA3.1 or RACK1 into HEK cells and used the membrane-impermeable antagonist ([3H]NMS) binding to measure cell surface receptors after 5, 15, 30 and 60 minutes of carbachol stimulation (Fig. 2B).
To measure cell surface PTPRZ1 levels in sh Z1-transduced SCLC cells, we used flow cytometry (FACS).
NADH is an impermeant substrate and, therefore, can be used to measure cell surface NADH activity with whole cells.
To measure cell surface VEGFR-3, a fraction of the lysates was precipitated with streptavidin agarose beads (Life Technologies).
More suggestions(15)
estimate cell surface
measuring elevation surface
measuring cell wall
measuring road surface
measuring cell fluorescence
measuring cell migration/invasion
measuring cell count
measuring thrombus surface
measuring cell density
measuring cell death
measuring cell apoptosis
measuring cell proliferation
measuring root surface
measuring cell uptake
measuring cell mass
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