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In addition, for MCF-7 cells we verified the utility of this assay for measuring cell number by comparison with DNA content measurements and with cell counts by hemocytometer (data not shown).
Cells were treated with nine concentrations of drug for 72 h before measuring cell number relative to controls [12].
We first checked for defects in cell proliferation by measuring cell number in Drosophila S2 cells upon RNAi-mediated depletion of genes of interest.
Methodologically, the activity of STI571 in combination in these studies was assessed by the MTT assay, which is strictly a technique for measuring cell number and not cell kill (Carmichael et al, 1987).
The utility of the CV assay in measuring cell number was verified both in MCF-7 cells and in combination with immunodetection plate assays for GH3/B6 cells [ 17].
First, we studied the effect of BRAT1 silencing on cell growth by measuring cell number and the MTT (3- 4,5-dimethylthiazol-2-yl -2,5-diphenyltetrazolium bromide, a yellow tetrazole) assay.
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Bacterial colonization was monitored from 0 to 14 days in three concentric portions of the sterilized clods (outer, intermediate and inner) and in the surrounding non-sterile soil, by measuring cell numbers and substrate-induced respiration (SIR).
Therefore, we performed cell proliferation studies in CA9-22 by measuring cell numbers (Trypan Blue exclusion), DNA synthesis (H-thymidine incorporation), and PCNA expression (immunohistochemistry).
In addition, both inhibitors, used individually, suppressed oncogene-induced cell hyperproliferation, whereas empty vector-transduced cells were not significantly affected this was observed both by measuring cell numbers and the fractions of BrdU-incorporating cells.
After measuring cells number, the titanium disk was divided into nine areas.
Resazurin, a soluble dye that is reduced to highly fluorescent resorufin in proportion to the metabolic activity of a cell population, is a valuable, noninvasive tool to measure cell number.
More suggestions(15)
measuring cell count
measuring cell proliferation
measuring cell mass
measuring cell fluorescence
measuring cell migration/invasion
measuring cell density
measuring copy number
measuring cell death
measuring cell apoptosis
measuring cell uptake
measuring cell growth
measuring cell viability
measuring cell type
measuring cell surface
measuring cell adhesion
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