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The other line of emerging technologies for measuring cell mass relies on optical interferometry.
Secondly, the current method of measuring cell mass by subtracting the Avicel residue weight (determined by the aforementioned enzymatic digestion procedure) from the weight of the cell-Avicel pellet, is time-consuming.
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However, it must be recognized that there is a need to develop better tools for measuring beta cell mass and that using insulin secretion to determine functional beta cell mass is only partially informative.
To measure beta cell mass, three randomly selected insulin stained pancreas sections from each mouse (n = 3) were imaged.
Capacitance readings were used to accurately measure viable cell mass profiles over five cycles.
We have therefore measured alpha cell mass in a larger series of non-diabetic and type 2 diabetic subjects whose beta cell mass was reported recently [ 7].
The periodic sampling of A. resinae ZN1 broth from the fermentor was not allowed to measure the cell mass accurately because of the flocculation of cell mycelia and aggregation [ 24].
Subjects The study, conducted according to the regulations of the local ethics committee, was performed on pancreatic samples obtained at autopsy (within 12 h of death) of the subjects in whom we previously measured beta cell mass [ 7].
These technologies include microfluidics systems, such as the microscale cantilever described in (Son et al. 2012), which is capable of measuring single-cell mass changes as a function of cell cycle progression, and high-content automated imaging systems, which are being used to quantify phenotypic variability (i.e., growth rate, migration, etc).
When we measured β-cell mass, we found that it was intact (Figure 1SE).
It would be enormously helpful to be able to measure β-cell mass in humans with noninvasive techniques.
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