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The cell viability of N Chitosan-DMNPs was evaluated by measuring cell growth inhibition using a 3- 4,5-dimethylthiazol-2-yl -2,5-diphenyltetrazolium bromide (MTT) assay (Roche Molecular Biochemicals, Mannheim, Germany) compared to DOX as a control.
Despite these massive challenges there has recently been a renewed interest in measuring cell growth and several methods have emerged.
To date, studies on the effects of these algicides on HAB species have mainly focused on the biocidal efficiency, by measuring cell growth, pigment content, and photosynthetic efficiency.
The traditional and ubiquitous method for measuring cell growth involves using impedance counters to acquire size distributions combined with tedious mathematical analysis of the population level statistics [ 3].
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Broth samples (1 mL) were collected at 24-h intervals (before and after the glucose feeding) to measure cell growth.
After incubation at 37°C for 16 h, the absorbance of each culture was read at A600 to measure cell growth.
A spectrophotometer was used at 600 nm to measure cell growth of E. coli, P. pastoris, and L. paracasei.
To define the working conditions for the TubeSpin® bioreactor 600, we measured cell growth and environmental culture conditions such as pH and dissolved O2 and CO2 concentrations over a range of shaking frequencies and working volumes using a stable CHO-derived cell line expressing a human IgG antibody.
To measure cell growth an additional sulphorhodamine b (SRB) assay, which estimates the protein content of cells, was performed [58].
We measured cell growth by direct counting each 24 h during 4 days and found that cells expressing isoform 1 or 2 grew more rapidly than control cells expressing EGFP alone.
To measure cell growth, CCK-8 (Dojindo Molecular Technologies Inc., San Diego, CA, USA) was used according to the manufacturer's protocol, and cells were measured at a wavelength of 540 nm in an enzyme-linked immunosorbent assay plate reader (EL800, Bio-Tek Instruments Inc., Winooski, VT, USA).
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