To further investigate the relationship among these enzymes in affecting cell proliferation, we measured cell counts in S2 cells having different combinations of knockdown factors.
To measure cell proliferation, a Cell Counting Kit (CCK; Dojindo, Kumamoto, Japan) was used.
To measure cell survival, we counted the frequency of TUNEL+ cells among E-cadherin+ cells.
Cell attachment was measured by cell count, DNA content analysis, and scanning microscopy.
Only PENTA 2002 measured CD4 cell count (absolute, cells/mL) from baseline to 48 weeks.
Hoechst was utilized to measure total cell count and nuclear shape.
Full blood aliquots were used to measure red cell count, leucocyte formula, platelet count, hemoglobin, hematocrit, and mean corpuscular volume.
Proliferation of the cell lines was measured by Cell Count Reagent SF (Nacalai Tesque, Kyoto, Japan) according to the manufacturer's instructions.
Cells were incubated in the presence of increasing concentrations of gefitinib for up to 8 days, and growth measured by cell count relative to untreated samples.
We stained dissociated RSNs with NeuN and LIVE/DEAD to measure neuronal cell count and death, respectively, at various stages after SCH.
