Proliferation was examined after 48 h by measuring cell phosphatase activity based on the release of paranitrophenol (pNPP) (Sigma) measured at OD 405 nm (Fluostar optima; BMG labtech, Champigny-sur-Marne, France).
In the current study, we compared two methods for measuring cell migration, both based on introducing a 'scratch' to a cultured monolayer of cells (adapted from a method previously reported for keratinocytes (Cha et al, 1996)).
Similar to the MTT, the 2,3-bis- 2-methoxy-4-nitro-5-sulfophenyl -2H-tetrazolium-5-carboxanilide (XTT) measures cell viability based on the activity of mitochondria enzymes in live cells.
The MTT assay is a sensitive, quantitative colorimetric assay that measures cell viability based on the capacity of mitochondrial succinyl dehydrogenase in living cells to convert the yellow substrate (3- 4,5-dimethylthiozol-2-yl -2,5-diphenyltetrazolium bromide, MTT; Sigma) into a dark blue formazan product.
Specific serum antibodies against hgp100 and htyr were measured using cell based flow-cytometric assays.
The effects on cell growth were measured in cell based assays using the fixed molar ratio design and the median effect principle.
In order to test the efficiency of this method for quantifying anaerobic rumen fungi, it has been attempted to evaluate this method in in vitro conditions by comparing with an assay based on measuring cell wall chitin.
(21) The principle of measuring cell volume in our sensor is based on the fact that cells are electrical insulators at low frequencies.
Many methods have been developed to measure cell proliferation including those based on direct counting of viable cells, measurement of metabolic activity and cellular DNA content.
In our laboratory, we recently developed a new technique to measure cell proliferation that is based on infrared spectro-photometry and the density of cell culture medium.
