Thus, our results indicated that knockdown of ATP1A1 specifically sensitized cells to aurilide B. Next, to determine whether a Na+/K+ ATPase inhibitor could mimic the effect of ATP1A1 knockdown on aurilide B sensitivity, we measured cell viability upon treatment with both aurilide B and ouabain, a well-known Na+/K+ ATPase inhibitor that acts by targeting α-subunits12, 13.
To measure cell growth an additional sulphorhodamine b (SRB) assay, which estimates the protein content of cells, was performed [58].
First, we compared CD4 T cell and APC activation in CL patients and healthy subjects by culturing PBMCs with pLAg and measuring CD4 T cell, B cell and DC activation markers.
We have measured B cell numbers and memory B cells numbers and relative percentages in adults in Blantyre, Malawi to see how far previously described HIV-associated abnormalities are present in our population of adults and how these abnormalities are changed after anti-retroviral therapy.
The PV parameters of the two newly measured J-V curves of cell B in Fig. 3b are given in Table 1, too.
The differences in kinetics and absence of correlations between memory B cells and plasma antibodies in this study are in line with the independence between the two B cell subsets and emphasize the importance of measuring memory B cells directly when determining the induction of memory by PHiD-CV.
Notably, no SIV Env-specific antibodies were elicited by the RhCMV/SIV vaccine as compared to the DNA/Ad5 regimen suggesting that measured B cell responses are unlikely to contribute to the protection in these animals.
Protein expression of AT1R and ETAR was measured on T cells, B cells and monocytes by flow cytometry.
FACS methods were employed to: a) measure the fluorescence intensity in each cell b) detect and count the number of fluorescentprotoplasts in large populations (10,000 protoplasts).
For in vitro experiments, we purified B cells from the spleens of TLR4WT or TLR4P712H mice, and then measured B-cell proliferation, secretion of IL-10 and production of IgG3 in response to the following agents: LPS alone, αIgM alone, mixtures of LPS and αIgM, and LPS conjugated to αIgM.
