Sentence examples for measuring autophagy activity from inspiring English sources

Instead, a combination of assays is required for measuring autophagy activity.

Monitoring LC3-II conversion, LC3 distribution or its flux through the autophagy pathway are the gold standards for measuring autophagy activity and are widely used in the field.

Mice transgenic for GFP-LC3 have been generated and used to monitor autophagosome content in the nervous system, which also has the same caveats for measuring autophagy activity described above.

In order to parameterize our model, we measured autophagy activity steps in MCF7 breast cancer cells, at 3 hours under full medium (FM) conditions (basal autophagy), and at 3 hours of ND conditions (activated autophagy).

LC3 analysis is now widely used to evaluate autophagy activity.

This robust and sensitive method measures autophagic flux rather than autophagosome number and is an important addition to the autophagy researcher's array of tools for measuring autophagy.

To measure autophagy, cells were stained with acridine orange (AO) to detect vesicular organelles characteristic of lysosomal activity.

The science has come a long way since the 60s and researchers such as Simon can now measure autophagy by tracking the flow of labelled molecules associated with the process.

To measure autophagy, we performed Western blot analysis to measure LC3 cleavage and p62/SQSTM1 expression [23].

To validate the assay, we measured autophagy in cell lines and mouse splenocytes deficient in autophagy.

The level of LC3 lipidation has long been a reliable measure of autophagy activity in vivo (Klionsky et al., 2012).