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We modelled N mineralization potential by measuring accumulation of inorganic N (KCl extractable NO3− and NH4+) in response to sustained flooding in soils from a small intermittent stream in semi-arid, north-west Australia.
Following stimulation, glucose uptake was evaluated by measuring accumulation of 3H-labelled 2-deoxyglucose for 10 min, with 14C Mannitol as extracellular space marker[3].
Potency of low-dose RBV to suppress LPS induced NO release was evaluated by measuring accumulation of nitrites in the culture medium.
After the treatment, cells were collected and the cell extracts were prepared for analysis of proteasome inhibition by measuring accumulation of ubiquitinated proteins.
In the present study, we analyzed the autophagic responses of human prostate cancer cells to VPA treatment by measuring accumulation of LC3-PE conjugates and formation of LC3 puncta.
Additionally, measuring accumulation of iron and an impaired ISC dependent protein activity in mutants in which Sco1, Sco2 or Hol1 have been knocked out would provide evidence for the involvement of these proteins in ISC biogenesis.
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We then measured accumulation of amyloid precursor protein (APP) in the corpus callosum of SIV-infected macaques as a marker of neuronal dysfunction to verify that expression of the Mane-A*10 allele also conferred protection against neuronal damage [16].
All three studies only measured accumulation of the non-cross-linking AGE CML.
We also measured accumulation of oxidative damage (protein carbonylation and lipid peroxidation), telomere length, liver somatic index and condition factor that all indicate more long term changes in health and aging.
GARFTase inhibition was confirmed by an in situ GARFTase assay in R2/hPCFT4 CHO and R1 11-PCFT4 R1 11-PCFT4wHeLa measures acellslation of the GARFTase product [C]formyl GAR in the presence of [C]glycine, and by direct assay of recombinant murine GARFTase in the presence of the monoglutamyl drugs.
The traveling ratio has previously been used to measure accumulation of PolII within the promoter region and is defined as the ratio of total PolII density at the promoter (−30 to +300 bp) relative to the total PolII density in the gene body.
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