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Such experiments produce quantitative time-course data measuring absorbance and fluorescence.
The new design multiplexes the original light sensor in time to serve the dual purpose of measuring absorbance and fluorescence.
The concentration of each library was determined by measuring absorbance and confirmed for downstream processing if the OD 260/280 was approximately 1.8.
This was accomplished by the addition of chloramphenicol (a bacterial translation inhibitor) to the bacterial cultures and measuring absorbance and fluorescence over time.
The quantity and quality of isolated RNA were tested by measuring absorbance and capillary gel electrophoresis using the 2100Bioanalyzer and RNA 6000 Pico Kit (Agilent Inc., Santa Clara, CA, USA).
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A dedicated data acquisition system is designed and fabricated using a LPC2106 microcontroller with light emitting diode (LED) as source and photodiode as sensor to measure absorbance and to calculate the calcium concentration.
A clear relationship was observed between the measured absorbance and increasing units of chitinase.
A Biotek Synergy 2 microplate reader running Gen5 software was used to measure absorbance and fluorescence of biological cultures.
These samples were also used to measure absorbance and analyzed for elemental composition using energy dispersive X-ray fluorescence (XRF) at Cooper Environmental Services (Portland, OR, USA).
Subjecting the dual-labelled nested PCR product to the EIA, resulted also in a detection limit of 10-2 Ifig(fig. 4B) and there was a linear relationship between the measured absorbance and the tenfold dilution series.
pH on the total hydrogen scale was calculated from the measured absorbances and the thymol blue dye parameters (pK2, e1, e2, and e3) at the cuvette temperature and a salinity of 34.1, using the algorithms of [38].
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