These measurements were confirmed using a radioactive charcoal assay measuring the release of P-labelled Pi upon GTP hydrolysis.
RNA quantity was measured spectrophotometrically and its integrity was confirmed using 1.2% agarose-formaldehyde gel electrophoresis [ 79].
The accuracy of expression measures based on PM-only design was confirmed using exogenous spike-in controls, and by PM-MM analysis of genes on chromosome Ib (blue vs. gray in Additional File 1).
The amount of RS in the final products was confirmed using the AOAC 991.41 method, which measures total fiber (and the most reliable method to measure RS4).
A signature of room temperature ferroelectricity in the films was measured by piezoresponse force microscopy and was confirmed using angular dark field scanning transmission electron microscopy.
Moreover, children's self-reported non-smoking status was confirmed using an objective measure of smoking.
The sampling adequacy of the food group variables for factor analysis was confirmed using the Kaiser Meyer Olkin measure.
RNA concentrations were measured using a Qubit fluorometer, and the integrity was confirmed using a 2100 Bioanalyzer (Agilent Technologies).
Cellular viability was confirmed using a CyQuant NF assay, which measures cellular content via fluorescent dye binding (data not shown).
This was confirmed using TBR1 null mice.
