Exact(3)
Instead of measuring gene expression levels across individuals, allele-specific expression (ASE) analysis measures transcript abundance within an individual 43.
In order to determine whether our technique quantitatively measures transcript abundance, we compared our EST library to two SAGE libraries of R1881 treated LNCaP cells [ 15, 16].
The expression is evaluated in three different measures: transcript expression accuracy (Theta), transcript within-gene relative proportion accuracy (WGE-True) and inter-replicate consistency (WGE-Inter).
Similar(57)
Consistent with this, systemic dFOXO transcriptional activity, assayed by measuring transcript levels of multiple direct downstream targets of dFOXO in whole bodies, was significantly decreased in esgGAL4>ndi1 flies.
To elucidate the roles of SNB and OsIDS1 in controlling flowering time, we measured transcript levels of the previously identified floral regulators in OX plants.
Traditional cDNA or oligonucleotide microarrays represent a second extensively used method to measure transcript expression levels.
Here, we measured transcript levels of control transfected or HIF-1α-targeted pulmonary epithelia.
Next, we measured transcript levels of the enzymes involved in glutamine metabolism.
Quantitative realtime PCR to measure transcript levels was done as described previously [20].
DNA microarrays are high throughput technologies aiming at measuring transcript abundance for thousands of genes simultaneously [17].
The measured transcript abundance was normalized to the level of POLR2A (RNA Polymerase II) for all samples.
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