In addition to depth resolved changes of tissue scattering, OCT measured tissue movement over more than 40 μm with a few tens of a nanometer resolution.
This index is derived from water, collagen and lipid content, combined with a tissue scattering parameter, and is measured using time-resolved transmittance spectroscopy.
Our previous study [ 13] showed the potential of utilizing multiple SFR spectra measured by different fiber diameter probes to characterize the tissue scattering properties without any a priori information about the tissue PF.
DOS signatures are used to measure tissue hemoglobin concentration (total, oxy-, andeoxy-formsms), tissue hemoglobin oxygen saturation (oxy-hemoglobin relative to the total hemoglobin), water content, lipid content and tissue scattering.
By measuring the scattered light at multiple wavelengths within the near-infrared frequency range, one can recover hemoglobin (oxygenated, HbO, or deoxygenated, HbR), water and lipid concentrations as well as the tissue scattering properties.
The strength of volume, however, also varies with the tissue scattering coefficient and the number of moving scatters.
This provides greater signal penetration of tissue through reduced light absorption and tissue scattering.
This is because tissue scatters light significantly.
These results are mapped into the scattering coefficient and anisotropy of scattering, providing a new approach for measuring tissue optical properties using confocal microscopy.
In brief, the instrument combines frequency domain photon migration (FDPM) and continuous-wave near-infrared spectroscopy (CW-NIRS) measurements to determine the optical scattering and absorption spectra (650 to 1,000 nm) of the measured tissue.
