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Cellulase activity was determined by the method of Ghose (1987) that measures the release of detectable reducing sugars removed from filter paper (FPase).
One such method measures the release of endogenous enzymes (e.g., lactate dehydrogenase) in the supernatant during the CTL-mediated cytolysis of target cells [4].
The cytotoxicity was assessed by an independent method using ToxiLight BioAssay Kit (Lonza, Rockland, ME), which quantitatively measures the release of adenylate kinase (AK) from damaged cells [45].
Additionally, treatment of Ae. aegypti SGE with serum from sensitized mice caused no reduction in apyrase activity as judged by an in vitro biochemical assay that measures the release of phosphate from adenosine triphosphate (Methods S3; data not shown).
The AR reaction measures the release of label from GS-[P]AMP.
Proteoglycan loss was assessed by using the dimethylmethylene blue (DMMB) assay, which measures the release of sulfated glycosaminoglycans (GAGs).
Similar(47)
The standard assay is the Dahlquist method which incubates the biopsied tissue together with the appropriate substrate and measures the released glucose.
This apparatus burns the biomass at 730°C and measures the released CO x and NO x by infrared absorption.
These measurements were confirmed using a radioactive charcoal assay measuring the release of P-labelled Pi upon GTP hydrolysis.
The headspace analysis was performed to measure the release of VOCs from rubber granulate under warm weather conditions.
Active denitrification was demonstrated by measuring the release of 15N in N2 to the headspace from added 15N labeled nitrate.
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