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According to Telford et al. (1990) electrical log is the oldest geophysical logging technique that measures the formation electrical properties in the vicinity of the borehole.
To elucidate a role for Notch signaling in angiogenesis, we employed an assay that measures the formation of tubular capillary-like structures by endothelial cells growing in a three-dimensional matrix.
To quantitate the ATP-dependent catalysis of nucleosome spacing, we devised a nucleosome spacing index that measures the formation of distinct di- and tri-nucleosome MNase digestion bands.
For example, the radioactive or colorimetric assay measures the formation of a radioactive product and a ureido product, respectively, without yielding sufficient information about its identity.
The nuclease protection assay measures the formation of a ternary complex by the ability of EF-Tu to protect the 3′ end of the tRNA with the covalently attached [H]Leu from digestion by RNase A [ 45].
Briefly, this discontinuous colorimetric assay measures the formation of urea-containing compounds (e.g., citrulline, urea, methylurea, etc).. Steady-state kinetic parameters were determined with variable amounts of the substrate in reaction buffer (60 μL final volume).
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For example, in the ping pong mechanisms that are shown above, rapid kinetic measurements can follow the release of product P and measure the formation of the modified enzyme intermediate E*.
Cytotoxicity was measured using a 96-well format and a standard WST reagent assay by measuring the formation of red formazan compound at 500 nm using a plate reader.
TyrBm activity was determined by measuring the formation of 2-hydroxy-p-benzoquinone (HPB) from HQ, in the presence of H2O2 or L-dopa.
Using [α-32P]-ATP and autoradiography to measure the formation of AMPylated EfFIC (denoted AMP*EfFICWT), we observed that EfFICWT has conspicuous autoAMPylation activity in the presence of Mg2+ (Fig. 1f).
In addition, these newly synthesized compounds were evaluated for in vitro inhibitory activity against commercial enzyme xanthine oxidase (XO) by measuring the formation of uric acid from xanthine.
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measures the world
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measures the government
measures the performance
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