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The Illumina DASL™ expression microarray platform was selected as the biomarker measurement method, because it measures the expression of gene targets using paraffin tissues [17] [19].
The NanoString Prosigna assay [ 26] measures the expression levels of 50 target genes plus eight constitutively expressed normalization genes [ 15, 27, 28]. Assay controls are included to ensure that test samples and the test process meet pre-defined quality thresholds.
The NanoString Prosigna™ (NanoString Technologies, Seattle, WA, USA) assay measures the expression levels of 50 target genes (including HER2) plus eight constitutively expressed normalization genes (PAM50) to classify a tumor as one of four intrinsic subtypes (luminal A, luminal B, HER2-enriched, and basal-like), which have been shown to be prognostic [ 117, 118].
The perfect match probe has a sequence exactly complimentary to the particular gene and thus measures the expression of the gene.
Using a "DNA chip", which measures the expression of large numbers of genes simultaneously, researchers identified a signature of 51 genes, allowing TB to be distinguished from both latent TB and other diseases that mimic the disease's symptoms.
Each experiment measures the expression levels of thousands, if not all, of the genes in the cell simultaneously.
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Therefore, we measured the expression level of these factors after manipulating the expression of Lrrc75a-as1.
In addition, we measured the expression of gp91phox, a major component of NADPH oxidase.
We also measured the expression levels of type I IFNs and type II IFN (IFN-γ).
We measured the expression of the transcription factor Bcl-6, a regulator of Tfh cell differentiation17.
Then, we measured the expression of steatosis target genes of PPARγ2.
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