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The GPA assay measures stem cell or precursor erythroid cell mutations expressed in peripheral red blood cells of MN heterozygous subjects, identifying NN variants, which result from loss of the GPA M allele and duplication of the N allele, and N phi variants, which arise from gene inactivation.
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We further measured stem cell pluripotency marker SOX-2 at 2 weeks after SCI.
By designing clonogenic assays to measure stem cell differentiation in vitro and serial transplantation assays to study stem cell repopulating ability in vivo, Till and McCulloch defined the hallmark characteristics of stem cells.
We previously established two other functional assays to measure stem cell activity in the prostate.
However, while the NSA is an appropriate method to identify stem cell activity, we contend that the enumeration of spheres is not appropriate to measure stem cell frequency or expansion rate as doing so results in an overestimation [20], [21].
Differentiaton can be used to measure stem cell quality.
Osteogenesis was determined by measuring stem cell tissue production and gene expression in vitro.
Furthermore, the modulation of these targets also explains the link between the chemical screens measuring stem cell differentiation ['Stem Cell Differentiation (Cell count)' assay] and DNA methylation [by 4,6-diamidino-2-phenylidole staining in 'Histone Modification (DNA methylation)' assay].
Further work is possible to study later stages, apply other methodologies to study gene expression, and to compare to other microarray experiments measuring stem cell gene expression changes with differentiation.
A total of twelve questions measured attitude to stem cell use in the questionnaire.
By evaluating in vitro measures of the cancer stem cell phenotype, such as tumoursphere formation and stem cell marker expression, they showed that the stem-like population was enhanced.
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