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It is similar to the [H] based assay in that this assay also measures proliferation, but the proliferation of CD4+CD25− T cells is measured by the decrease of green fluorescence from CFSE dye when cells divide [ 26].
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Flow cytometry measurement of the SPF is perhaps the most clinically validated method for measuring proliferation.
Here, we report a simplified method for measuring proliferation of malignant B cells in patients with chronic lymphocytic leukemia (CLL).
We measured proliferation (3H-thymidine incorporation) and collagen secretion (protein-binding assay, collagen type II-ELISA and immunocytochemical staining of pericellular collagen types I, II and IX).
Measured proliferation rates were consistently described.
First, we measured proliferation of our cell lines in the presence of both inhibitors by MTT proliferation assay.
To measure proliferation, cells were seeded in 6-well plates and transfected with siRNA on the following day at around 30% confluence.
In experiments measuring proliferation, BrdU (0.8 mg/ml) was added to the drinking water to the mice for three days before sacrifice.
CFSE counts were gated on KJ1-26+ cells and CFSE dilution was analyzed using flow cytometry to measure proliferation due to T cell activation.
In order to measure proliferation rate, 3T3 Dohhflox/flox; Crepos.
The cell Proliferation Reagent WST-1 (Roche) was used to measure proliferation plating 3000 cells/well.
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