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Four measures per image were obtained at 0, 90, 180 and 270° to estimate intima and media thickness (IMT).
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For stromule length measurements 30 stromules were measured per image.
On average, 1600 seeds were measured per image.
One hundred pore diameters were measured per image from three separate SEM images from three different batches of microspheres before and after treatment with EtOH NaOH for 2 min.
ImageJ was also used to measure the average crimp length within each picrosirius red-stained sample, based on at least 25 measurements per image.
By performing grinding trials, the method has been found to be able to accurately (with biggest relative error of 3.13% in comparison with the manually measured results) and quickly (with the measurement speed of 1.68 s per image) measure SSD depths.
One stiffness value per image was measured.
At least 10 myotubes per image were measured.
The sum of all pixel intensities per image was measured within the primary objects in both the Nogo-A and Apg-1 channel.
The line measurement tool was used to measure the thickness of the samples (all 10 scans per image were used for these measurements).
Therefore, the complexity of each metric was evaluated by measuring the mean runtime in seconds per image (s/img) over all datasets.
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CEO of Professional Science Editing for Scientists @ prosciediting.com