Your English writing platform
Discover LudwigSuggestions(1)
Exact(1)
We applied the TransCount model as an approach to eliminate unreliable data based on quantitative measures of template input in the initial amplification and for the threshold determination.
Similar(59)
If amplification failure is a measure of template variation, we find a much greater variability among plasmid-associated virulence factors.
As a result, the Ct values can therefore be used as a measure of template DNA and to quantify the relative amount of 3895 bp deletion compared to wild-type mtDNA.
Quantitative measures of DNA template quality determined by QFI-PCR were then compared with targeted NGS results using defined metrics across varying 'functional' FFPE DNA copy numbers to demonstrate DNA quality and input thresholds that supported accurate variant calling.
Serial dilutions of a known quantity of template for each gene were used to measure the copy numbers.
Threshold cycle (CT) values were recorded as a measure of initial template concentration.
Furthermore, we measured the performance of template extraction, stack create and update and showed our method could process templates in a sufficient short time.
Prior to our development of SeqZip, we had attempted to use a RT-PCR-based triple-read sequencing method to determine exon connectivity between Dscam1 alternative splicing regions 4, 6, and 9. To measure the extent of template switching, we generated four RNA transcripts corresponding to distinct isoforms.
The copy number of the assayed repeats in each sample's genome was measured as the ratio of template amount of the repeat to the template amount of single copy gene amplicons.
In our work, we address this issue and propose a modified Bloom filter based framework which provides all the desirable security measures of a biometric template protection scheme.
In the first part of this work different measures of similarity in template selection were explored and methods for homology modelling, docking and refinement of aminergic GPCR-ligand complexes were developed and evaluated by comparing models of the D3-R/eticlopride complex with the crystal structure.
Write better and faster with AI suggestions while staying true to your unique style.
Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com