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"We will bring more detail on the settlements and on our approach to addressing two states, and they will bring what they expect to get from the Arab world, meaning measures of normalization, and from the Palestinian side steps on security and moves to end incitement," he said.
However, these measurements were performed at just one time point, and not compared to other independent measures of normalization.
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Yet political engagement risks or even demands a measure of normalization.
A mean quantity was calculated from triplicate PCR reactions for each sample, and this quantity was normalized to two similarly measured values of normalization genes (glyceraldehyde-3-phosphate dehydrogenase and β-actin).
The outputs of EGFP and GLuc were correlated (Fig. 1D), indicating that GLuc was an appropriate measure for normalization of transfection efficiency.
Rad mRNA level was assessed by real-time quantitative RT-PCR as previously described [7] and 18S level was measured for normalization of variations in RNA input and cDNA synthesis.
As an internal control β-actin was measured for normalization and quantification of c-Myc expression.
Table 5 presents an extract from the different collected performance measures after the process of normalization.
This suggests that more elaborate measures of tobacco de-normalization are needed.
We therefore recommend developing and including well validated, comprehensive measures of tobacco de-normalization in future population surveys.
1−π0 thus estimates the number of regulated genes, which we use as quality measure for evaluating appropriateness of normalization.
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