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Here we have proposed three simple steps to meaningful measures of bacterial metabolic activity based on the autofluorescence of NADH.
Microbial community structure and functional capacity were measured both before and after wetting (21, 49, and 77 days post-wetting) using terminal restriction fragment length polymorphism measures of bacterial and fungal communities, and quantitative PCR of nitrogen cycling genes.
Differences in bacterial microbiota were assessed by standard ecological measures of bacterial diversity.
Screening analysis by T-RFLP showed a significant difference in three different measures of bacterial diversity between the microbial communities of subjects with SAR compared with nonallergic subjects.
These studies should include 16S sequencing for bacterial identification; metagenomic sequencing and measures of bacterial gene expression; and investigation of relationships of these measures to host gene expression and genotype.
While outcomes for individual patients are most important from a clinical point of view, an ecologic perspective is also useful to assess the relationship of aggregate antimicrobial drug use to aggregate measures of bacterial resistance.
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The efficiency of DNA extraction was determined by the measured amount of bacterial DNA in each sample as compared to the estimated amount of bacterial DNA before DNA extraction as a reference (100%).
Incubation then took place for a period of 6 to 9 h, and bacterial growth was determined by monitoring optical density as a measure of bacterial biomass.
The water vapour and carbon dioxide transport properties have been measured of bacterial (isotactic) poly 3-hydroxybutyrate) (ipoly 3-hydroxybutyrateth synthetiPHBandctits PHblendsB) and poly(epichlorohydrin) (PECH) have been measured.
Samples of liquid medium were periodically removed aseptically for measuring of bacterial growth (OD600 nm) and pH using UV visible spectrophotometer UV-16011, SHIMandU) and pH meter (3510 pH meter, JENWAY, Bibby Scientific Ltd., UK), respectively.
The OD595 was determined at 12 h after bacterial challenge as a measure of bacterial growth.
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