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On the other hand, large contigs are a rarity, making the usual measures of assembly quality almost useless.
Standard measures of assembly quality focus on assembly size and fragmentation, which are desirable for large whole genome assemblies.
Measures of assembly correctness were obtained using our scoring functions, all five scores for the Arabidopsis assemblies are shown in Figure 1.
Additionally, the contig N50 and other measures of assembly continuity and quality improved (contig N50: increased from 1056 to 2057 bp; mean contig length: increased from 658.19 to 868.98 bp).
While statistics such as number of contigs, percent of contigs > 500, and max contig size can be used to evaluate assembly completeness, they are poor measures of assembly correctness.
Conversely, assemblies with low scaffold N50 lengths may excel in one or more specific measures of assembly quality; for example, the Ray snake assembly ranked 9th for N50 scaffold length but ranked 1st in the two COMPASS metrics of coverage and validity.
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Contig size is one measure of assembly utility.
> -wrap-foot> sizefold sise is another measure of assembly utility.
By breaking contigs at misjoins, the corrected N50 provides a better measure of assembly quality.
This metric provides an alternative measure of assembly completeness and accuracy when the closest finished genome is too divergent.
Using custom settings with the Celera Assembler produced assemblies with the smallest overall assembly size and highest average ortholog hit ratio, a measure of assembly quality (Table 1, see Annotation).
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