Nevertheless, the relationship between qHTS and ITC measures of affinity is significant, and does not deviate significantly from the line of identity.
However, the slope of the uptake curve at low P concentrations, α, and the ratio between Vmax and K0.5 (VmareK0.5) are better measures of affinity than K0.5.
Materials and methods: Similarly to the Ligand Efficiency (LE) index, we explored the usefulness of the Ligand Specific Efficiency index (LSE), which we defined as the ratio between affinity (expressed as e.g. pIC50 or pKd), and the logarithmic value of the experimental non-specific binding measurement, CHI IAM).[Jiang] LSE provides a measure of affinity, normalized to non-specific binding.
The measure of affinity is thus interpreted as the degree of stability or instability of boundaries against rapid face loss from topological events.
Results indicated that the measure of affinity for solitude was not a significant predictor of solitude preference in the natural environments; while solitude preference in the natural environments contributed to the formation of affinity for solitude, β = 0.269, SE = 0.062, t-value = 5.891, p < 0.001.
Also the slope, α, of the initial linear part of the uptake curve is a measure of affinity.
Hamming distances is used as a measure of affinity among receptors and co-receptors: the probability of an interaction depends on the number of matches.
Idh: isocitrate dehydrogenase; GxE: genotype-by-environment interaction; Km: the Michaelis constant, a measure of affinity; Vmax: the maximum reaction velocity at saturating substrate concentration.
When the results of Figure 4A were analyzed using a Schild plot, p A2, a measure of affinity of the antagonist for receptor, was calculated to be 7.87 from the dissociation equilibrium constant (KB = 1.35 × 10−8 M).
In the table headings, ka1 denotes on rate, kd1 denotes off-rate, KD indicates affinity, SD denotes standard deviation of KD measure, and n indicates the number of independent replicates in the measure of affinity for each version of the antibody tested.
This enzyme exhibits classic Michaelis-Menten kinetics (Lipscomb et al., 2004), i.e. where ν is the velocity of the enzyme reaction, Vmax is the maximum velocity of the reaction, K m is the Michaelis-Menten constant (a measure of affinity), and S is the substrate concentration.
