Even when we used a conservative measure of localisation (click within the mass boundary), we did not replicate the findings of Evans et al. (2013, 2016) that there are circumstances where radiologists can detect a mass above chance but not localise it.
For our second measure of AIS localisation, we used measures of the start and end positions of the AIS, as described previously [37].
2) Imaging and measuring co-localisation between transferrin and a label for endocytosed membrane, FM-143FX, to control for the (in our opinion remote) possibility of endocytic vesicles that are not detected by labelling of all plasma membrane proteins with sulfo-NHS-SS-biotin.
Abu Dhabi is not the only place in the region to be implementing localisation measures in the labour market.
In addition, we measured the area delimiting 30% of maximal fluorescence intensity as a measure of the localisation of a particular marker within each micro-epidermis (Fig. 1d f).
For our first measure of AIS localisation, we simply took raw fluorescence values along this line profile for both YFP and βIV-spectrin and calculated the Spearman correlation coefficient r between the two samples.
Objective exposure was measured by the localisation of the service in geographical zones with low exposure to immigration, medium exposure, or high exposure.
However, we are measuring HuHMFG-1 localisation and not MUC1 kinetics, and it is possible that antibody binding or crosslinking may increase MUC1 turnover as is sometimes seen for other antigens.
SP was assessed using standard questionnaires, and brain activation was measured using electroencephalography (EEG) and sLORETA source localisation (standard low-resolution brain electromagnetic tomography).
In the first, we obtained normalised fluorescence profiles for βIV-spectrin and YFP along the axon (Figure 1A, right), and simply calculated the Spearman correlation coefficient r between these distributions as a measure of YFP AIS localisation.
