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Among the most important distorting measures in image processing is the Peak Signal to Noise Ratio, PSNR [ 25].
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d, e Bond lengths from almost all peaks measured in image from before and after aberration-corrected images respectively.
Cell soma size and immunoreactivity were measured in Image J on overlaid projections of several consecutive images.
YFP fluorescence was measured in Image J along three lines drawn across each cell, as described in Supplemental Experimental Procedures.
The total and the full-length band intensities were measured in Image Lab (Bio-Rad, Hercules, CA) and normalized to the wild type (Fig. 1D-F).
Wing spot size, which is the area with visible melanin, was measured in Image J 1.31v software by two different workers.
Synaptic connectivity was analyzed in image stacks with voxel size 0.103 μm (x/y-axis)–0.3 μm (z-axis), whereas neurite territories were measured in image stacks with voxel size 0.206 μm (x/y-axis)–0.5 μm (z-axis).
The velocity, the distance from start to end point – as a straight line – (D) and the total track distance (T) were measured in Image J with the manual tracking plugin.
DOI: http://dx.doi.org/10.7554/eLife.03790.013 To characterize how NPC inheritance evolved as the cells aged, the total fluorescence of the Nup49-, Nup170-, and Nup82-GFP reporters was measured in image stacks through the entire volume of mother and daughter nuclei of telophase cells and plotted as a function of the age of the mother cell.
The velocity and dimensions of discharging liquid are measured in images by a point-tracking method.
R1 and R2 were measured in Image-J (Wayne Rasband, National Institutes of Health, Bethesda, MA, USA).
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